Input[or Upload] a couple of Locus or Primer-Pair Names
AGI convention name(i.e. At1g09530 or At1g09530.2) is allowed for Locus and genopp ID (i.e. At1g09530.2f291r61) for primer-pair name.
Each locus[or genopp ID] should be sperated by whitespace[ or newline] and # character can be used for comment line.
Select one dataset and Submit
Search Conditions
Target amplicon size (80 to 800 bp): between and bp
Non-specific binding free enegy of each primer (-9 to -7 kcal/mole):
forward primer is greater than kcal/mole and reverse kcal/mole
Numbers of same[or differnt] sizes of possible amplicons from genomic DNA:
There are same amplicons below and different amplicons
Numbers of same[or differnt] sizes of possible amplicons from similar cDNAs:
There are same amplicons below and different amplicons
Number of primer-pairs being displayed:

Primer dimerization scores between primers (CE,PA and PE)
forward itself(ff),reverse itself(rr),and forward and reverse(fr)
Consecutive 3'end(CE, 0 to 16):
ffCE is below and rrCE below and frCE below
Pair Any(PA, 0 to 8):
ffPA is below and rrPA below and frPA below
Pair End(PE, 0 to 8):
ffPE is below and rrPE below and frPE below